BIOL 311 Lecture Notes - Lecture 5: Herpesvirus Entry Mediator, Calmodulin, Immunocytochemistry
17 Sep 2016
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2. hybridomas can be cryopreserved, thawed, and propagated at will = will last forever! Biologie = a drug produced by cells. 1982 - first biologie developed - humilins: human insulin prokaryotic cell. 1990s - notice cancer cells have unique molecular signature (different!!) this related to cell surface proteins. Elisa - enzyme linked immunosorbent assay (not a microscopy technique) this technique is designed to quantify a particular protein of interest ie. biologics. 1. necrosis - pathological cell death ie ricin = murder . Pi phosphatadylserine ps normal cell is a happy cell - ps is in the inner leaflet early apoptosis > ps > flip flops to the outer leaflet label early apoptotic cells necrosis - membrane rupture. Brazilian wasp mp1 - type of poison which causes necrosis - pokes holes in cells not in all cells - it is selective for cells with ps and phosphatidyl ethanol on the outside leaflet > characteristic to some cancers.
