BIOL 311 Lecture Notes - Lecture 4: Confocal Microscopy, Propidium Iodide, Fluorescence Microscope
17 Sep 2016
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Images point by point end up with stereo-image multiple labels - triple label = 3 colors (we couldn"t do this until confocal microscopy) Point/laser scanning confocal microscope generates a very bright image takes a long time to image. Photobleaching (fluorochromes) generate heat b/c produces much heat, cells are dead. Advantages: can image in milliseconds designed to look at dynamic activities in living cells. Faster than the first one decreases photobleaching lower laser intensity better for dynamic processes. Can distinguish different types of skin cancers, such as basal carcinoma from melanoma. Point/laser scanning - art conservancy and forgery. How? analyze what is inside a painting (there are layers of paint) we are worried about the paint decaying. Fluorescence microscopy and associated techniques (came before confocal) arose in 1970s uses fluorochromes (fluorescent dyes) Confocal microscope = v expensive fl. microscope = inexpensive. Vital fluorescent microscopy - using a fluorochrome to monitor a change in a select cell physiol- ogy.
