BIOL 311 Lecture Notes - Lecture 2: Polarizer, Deconvolution, Fluorophore
Document Summary
Lecture 3 microscopy: the foundation of cell biology. 1675: anthony van leeuwenhoek came up with images of what cells look like via sketches, bacteria and protozoa in particular. 200 years: carl zeiss from germany, abbe equation, textile dyes. 1900s: two camps/theories for brain: reticular theory: the brain is not made of cells, rather a reticulum (ex. blood vessels, neuronal theory: neurons are cells: golgi. Resolving power of microscopes: two types: diffraction limited: resolution dictated by illuminated source (light, not diffraction limited: new (within last five years) Most of the time will be sent on color selection. Questions relevant to microscopy: what microscope to select, how do we prepare the tissue, live of dead? a. i. Resolution: theoretical limit of resolution: the actual best, which would be abbe"s. Equation: practical limit of resolution: less than the best, always present when using cells, cells are poor candidates for microscopy compared to geological specimens a. i.
