BIOL 311 Lecture Notes - Lecture 8: Affinity Chromatography, Glut4, Puromycin
19 Aug 2016
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Lecture 8: explain the following observations, cells injected with antibodies to clathrin were unable to endocytose. answer: clathrin is a critical part of receptor mediated endocytosis. Once the ligand-receptor containing vesicle endocytoses the clathrin eventually dissociates from the vesicle, becomes soluble in the cytoplasm, and then recycles so that it can form another clathrin basket as part of the receptor mediated endocytosis process. Also, oligomycin might not decrease the large, stored atp pool that might be fueling the formation of the endosomes. These cho receptors will respond to insulin in a wild-type fashion. This is discussed in class: cells use a variety of receptors and receptor mechanisms to accomplish cell signaling. As described in your textbook they include g protein-coupled receptors, cytokine receptors, receptor tyrosine kinases, tgf receptors, etc. etc. Answer: hydropathy plots can be used to predict if integral plasma membrane proteins such as this unknown receptor is a single or multipass protein.
