BCHM 426 Lecture Notes - Lecture 4: Western Blot, Fragment Crystallizable Region, Immunoglobulin Heavy Chain
Document Summary
Specificity only wants to see 1 band (ignore everything else) Sensitivity get enough protein to detect (get small amount of protein) Large glycoproteins responsible for binding antigens and interacting with (activating) immune cells. Supervariable region (tip top) has lots of recombination. Protein of interest inject into mouse immune response bleed animal take antibodies from blood get polyclonal antibodies (bind in more than 1 place on proteins since they have slightly different variable regions) (many sites) Nucleic acids (dna) has low immunigicity wont illicit immune response cant get antibodies. Agiment: solves the problem of not having an immune response. Pathogen associated molecular patterns (bacteria/viruses), which have structure on surface, which will elicit immune response when combined with your insertion that didn"t create an original response. Pamps: flags for pathogens (even if its non-pathogenic) Monoclonal one type of antibody forming cells fuse with tumor cells [hybridomas] and the hybrid screens for desired antibody that you know will bind.
