ANTH 333 Lecture Notes - Lecture 35: Laminar Flow Cabinet, Ancient Dna, Molecular Cloning
16 Aug 2016
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How do we get enough ancient dna (adna): dna cloning. Cut dna to be cloned with restriction enzyme. Mix together, grow up, cut back out o. Biochemical technique that can be done in about 45 minutes. Mimics dna replication inside a tube (exponential rate of replication) Need to know sequence of region you want to copy. Need a primer that is complimentary to one of the two strands. One primer on one side for one strand. One primer on the other side on the other strand. Seal into a tube: dntp, primer, buffer, mg, water, and the template dna to be copied. Used for all kinds of things: forensics, ancient dna studies, dna diagnostics, paternity testing, entertainment, epidemiology and health. Problems unique to adna o adna vs. modern dna. Sequences may be broken down to very small pieces and be in really bad shape or not the piece you"re looking for. Modern dna contamination is the most likely result.
