CHEM 3AL Lecture Notes - Lecture 5: Mass Spectrometry, Bitters
Column Chromatography
:watch vides ,procedure not in textbook )
-used for :
-Analytical :
figuring out what's in mix
-preparative :separation for future use of components
-Applications :
-
purity ,
environmental samples ,drug Testing ,Identification
-
cancer diagnostics
:
-Healthy
an -
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**not possible
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column
-bitters from Tuc because ↳Mass spectrometer )
moving down column vs .up
or Tw plate .
stationary Mobile Phase *separated by polarity ranting :
Phase (Sp )UWP )o
silica solvent in nr Most poutk
TW phase Tank .
1
silica solvent at
column packed by added from
you above
oh
O0
-H
0
0
,
move
polar
Column -down
tooth like fo
this §fbnww *compounds may be nose in polarity ...
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-collect different fractions in up .rxw tubes
#
ctanw )-spot samples LTLC )
-determine purest
*
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