Budding yeast- sacchromyces frogs cerevisiae: knowledge of a candidate cloning of the putative open, mutant strain that cannot grow at. Frog clone- the clone is likely to include only the open reading frame: more advanced knowledge on the frog. ** ^ all questions we can ask about the orf and the gene is encodes. Once the dna samples are loaded onto the gel, an electric current is applied to the gel. Dna is negatively charged due to all the phosphate groups in the backbone of dna. Thus, dna will move towards the positive electrode: as the pieces of dna move through the gel, they will meet with resistance. Larger pieces of dna will have more difficulty moving through the gel than smaller fragments. Thus, larger fragments will move slower than smaller fragments. This allows separation of all different sizes of dna fragments: electrophoresis is a common lab technique used to identify, quantify, and purify nucleic acid fragments.
