CHE 4341 Lecture Notes - Lecture 1: Myoglobin, Proline, Collagen
Document Summary
Can also be used to separate biomolecules. Separates proteins by their isoelectric points (= pi"s = ph at which the protein has no net charge) Can separate mixtures w/hundreds of different proteins. 1 - run ief gel in one dimension. 3 - can use to determine all proteins expressed in a cell. This is proteomics to separate by pi to separate by size. Used to detect viruses (aids, food allergens, etc) Blot = transfer from gel to membrane (nylon, nitrocellulose, etc) Western blot = transfer of protein from gel to membrane. Detect w/antibody targeted to protein of interest (immunoblotting) Aa monomers joined by peptide linkages = polypeptide strands. Aa sequence & location of s-s bonds. Polypeptide strands may form pleated sheets or helices. Interaction of aa close to each other. Form specific patterns ( pleated sheets or helices) 2+ polypeptides assemble to form larger protein molecules. Arrangement of multiple folded protein molecules (subunits) in a.
