BIOL 2905 Lecture 1: Probe microscopes
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You have just completed a microarray analysis to determine which genes are expressed downstream of a receptor X. You divide a population of cells in two. You add ligand to half the population and add nothing to the other half (your control). After 24 hours, you isolate the mRNA from the two cell populations and create cDNA from both sets of mRNA. You tag the cDNA from the control cells with a green fluorescent probe and tag the cDNA from the ligand-treated cells with a red fluorescent probe. You add both sets of labeled cDNA to a microarray chip & analyze the results. What can you conclude about a gene whose spot on the chip is red?
a. Receptor X signaling has no effect on expression of the gene | |
b. Receptor X signaling results in increased expression of the gene | |
c. The gene is not expressed in control or ligand-treated cells d. Receptor X signaling results in decreased expression of the gene |