BI236 Lecture Notes - Lecture 10: Ptk2, Pericentriolar Material, Spindle Apparatus
Document Summary
Antimitotic drugs > disrupts mitotic spindle of divided cells > anticancer drugs. Clochisiome > binds the gtp-binging domain ta the n terminus at the alpha and beta tubulins. Taxol > binds to microtules and stabilized them, causing a de ation of free tubulin subunits, arrests dividing cells in mitosis. treatment of lungs, ovarian, breast cancer (paclitaxel, abraxame) In vivo mt"s commonly originate from mtoc"s which is the site for initiation of assembly (nucleate) and anchors mts. Centrosome > an area in the cell which contains two centrioles and associated pericentriolar material. The walls of centrioles > composed of a set of triplet microtubules. Involved in baseball body formation for cilia and agella. Dna visualized with a uorescent stain (dapi) Ptk2 cells are a cell derived from male rat-kangaroo epithelial kidney cells. This cell line is used in biomedical research it is particularity popular as a model for mitosis; only a small number of chromosomes are easily visualized under a microscope.
