Biology 1002B Lecture Notes - Lecture 3: Northern Blot, Western Blot, Mental Floss
9 Apr 2016
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Maxwell: if you"re a photo-system (photosynthetic organism), want to gather as much light as possible. In a photoreceptor, the arrangement of rods and cones is attempting to mimic/harvest light to convey information. Mrna decay: decays after a period of time, so transcription is dependant. Transcript abundance: dependant on available mrna. 97% of total rna is ribosomal (those are the bands you see) Transfer to a membrane and probe (labeled radioactively to detect) Make a single stranded dna probe, which would hybridize to the mrna corresponding to that gene. They will stick together at the complimentary sequence. Since the probe is radioactive it can then be transferred to film. Use an antibody, which could be raised in a mouse, goat, etc. Antibody is going to bind with the presence of where the specific protein is. Reveals on western blot: (northern blotting?) Chlamydomonas cells shifted from growth temperature of 24 degrees to 40. 3 types of possible kinetics: constitutive expression.
