Biology 1002B Lecture Notes - Lecture 18: Apoptosis, Primase, Replisome
24 Aug 2013
School
Department
Course
Professor
Document Summary
Anti-parallel complementary base pairing allows for accurate replication. Replication bubble arises from two forks created at one origin. Both strands are replicated in the same replisome at the same time, very close together. Dna synthesis proceeds by adding a new base to the 3" oh of a properly-paired base. It is only possible to extend 3" ends. Rna primase synthesizes rna primers, which are extended by dna polymerase iii, and then dna polymerase i removes the primers and replaces them with dna. Both strands have polymerases reading from the 3" to 5" end. They are being replicated by the same rules. Replisomes replicate both strands at the same time by the same replisome. One strand is replicated continuously; the other strand is replicated discontinuously. The rna primer on the leading strand is unable to be replaced with dna by dna polymerase i because there is no 3" oh.
