Biology 1002B Lecture Notes - Lecture 3: Hybridization Probe, Functional Genomics, Jellyfish
24 Oct 2016
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Direct manipulation of an organism"s genome using biotechnology techniques. Using many techniques discussed previously: re digestion, ligation, cloning/expression vectors. Organisms that have undergone a gene transfer are called transgenic. For cloning/genomic library production: don"t need to express dna, lacz (multiple re site), ampr gene, origin replication. Expression vectors have regulatory sequences: sequences necessary to express gene in host organism, express in e. coli. Gene for the protein is inserted into an expression vector that contains regulatory sequences that allow transcription and translation of the gene. Eukaryotic dna contains introns: e. coli can not splice introns. Need to make cdna copy: reverse transcriptase, create cdna library, probe for gene of interest. E. coli has been genetically engineered to make insulin; commercial product is called humulin. Insulin regulates blood glucose: allows transport into cells, when absent glucose builds up in blood, starves cells. People who don"t produce enough insulin have diabetes. Further modifications to genes alter insulin properties.
