Biology 3338A Lecture Notes - Lecture 5: Germplasm, Rna, Syncytium

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Mutated inner mass cells are injected back into the inner cell mass of another embryo. Keep in mind these es cells are not determined, you can take them out and move them around and they will still form another embryo. So even if you have extra cells, it won"t matter. These cells communicate between each other, so the injected cells will become part of the embryo. But you don"t know where they will develop/ ie what tissue. The mutated ones confer resistance to all the cells (the entire embryo). So you have many of these embryos (injected with mutated bmp), you take them and inject them back into the ovary of a pseudopregenant female and hopefully you get the birth of these mice. They are called chimeric mice. so you don"t know which tissues are going to contain the mutated bmp. It"s a random process depending on where they are.

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