Biology 2581B Lecture Notes - Lecture 22: Knockout Mouse, Shuttle Vector, Selectable Marker
14 Jul 2016
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Produces large number of offspring in a short period of time. Cloning/transformation procedures are in place: genome sequence o o o. What should they model? o: mosaic determination when embryo formed by mitosis (c. elgans) each cell already assigned o specific fate. In some model organisms scientists can use targeted mutagenesis to construct animals bearing null mutations. Formation depends on es, if cultured cells are injected into different early embryo can contribute to tissues in mouse that develop from embryo. To generate: disrupt cloned gene of interest by inserting foreign dna into middle of gene. Then treat culture of es cells with altered gene. Some of cells take up altered clone gene and in small fraction homologous recombination allow altered gene to replace original gene. Cells now containing null allele are multiply in cell culture and injected into mouse embryos o o o o o o o o: different model organisms depending on what group you"re studying, different scales.
