Biology 2581B Lecture Notes - Lecture 5: Marbled Lungfish, Flow Cytometry, Schiff Test
11 Feb 2016
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Document Summary
Genome size the length of one set of genetic instructions in a compartment. 6 copies of 1 chromosome (n=6) genome size = length of 1. 6 different chromosomes (n=6) genome size = sum of length of copy each. Bp, kb (1000 bp), mb (1,000,000 bp), gb (1,000,000,000 bp) Bp (doubled stranded) vs. nucleotide (single stranded) Mass: 1 picogram of dna = 1gb. Sequence and assemble grow organism isolate its dna send it to be sequenced get millions of sequencing reads (100 nt) assemble on a computer by using overlaps to form contigs. Gel electrophoresis good for small pieces of dna (eg. - staining and imaging good for big genomes mitochondrial genome) Dna flow cytometry: isolate and stain dna (schiff reagent) measure fluorescence intensity of stain to determine amount of dna. Measures lot of dna quickly used to detect aneuploidy, apoptosis, and cell cycle.
