Biology 2382B Lecture Notes - Lecture 2: Ndc80, Cell Membrane, Interphase
11 Jan 2015
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Specifically, it is involved with vesicle transport in which its directionality along the microtubule is bi-directional. This involves taking a squid giant axon and extruding the axoplasm (basically the cytoplasm) out of it, which contains microtubules. You are able to see things moving in this prep and you can figure out how fast things move, what type of energy (atp) you need. Proteins are labelled in order to see what happens to them in the squid axons. This is done by injecting radioactive amino acids into the squid axons. Then a couple of nerve segments are cut with radioactive protein. You isolate this protein and run it on a gel (separating proteins in terms of size). Because these proteins are radioactive, you can place on a film to more clearly see the proteins separated in terms of size. Certain groups of proteins will be seen clustered together, meaning proteins tend to move together at a certain rate.
