Biology 2382B Lecture Notes - Lecture 1: Fetal Bovine Serum, Hayflick Limit, Trypsin
7 May 2019
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Cell: fundamental unit of life: studying cell allows understanding of complex functions, need to know normal state to fix abnormal problems. Hypothesis-driven experiments: isolate cells and examine in artificial enviro (in vitro) easy to control variables, identify the proteins that drive process. Cell culture: use to cells from organisms and have them able to grow/survive in vitro: isolate tissue by breaking down (stuck together by extracellular matrix, mechanical (cut w/scissors) Trypsin: chews of protein ends (protein makes cells stick) Edta: chelates ions (ions involved in cofactors for makes protein sticky) Get rid of ions proteins more sticky don"t function as well. Add trypsin (cleans ends of protein off: put into culture medium (contains things cells need: aa, nutrients) important additive is serum (blood derived product) Primarily use cow serum fetal bovine serum : insulin: triggers cells to uptake glucose, growth factors: initiate entry to cell cycle/divide, incubator: regulate temp, co2 pumped in cause interact w/buffer in media to maintain ph.
