Biology 2382B Lecture Notes - Lecture 1: Hayflick Limit, Cell Culture, Contact Inhibition
10 Mar 2018
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Requirements to study cells: tools and methods to isolate and maintain cells in vitro, how to view cells and separate organelles and proteins. Insulin to promote the uptake of glucose in the cells: growth factors to tell cells to remain alive and divide. Adherent cells: grown in a monolayer in a cell culture dish or tissue flask. Suspension cells: grown in spinner flasks on a magnetic stir plate with metal rod that spins inside or roller bottles that spin slowly to constantly tumble the media so the cells never settle: e. g. blood cells. Laminar flow hood: where we perform cell culture techniques to prevent bacterial contamination. Confluent monolayer: very packed and slow growing, exhibit contact inhibition (stop growing: to continue growth, trypsinize and passage/split cells. Cell line: transformed cells that are able to grow indefinitely (immortal: do not exhibit contact inhibition -> can grow on top of one another, often have chromosomes with abnormal dna sequences or additional chromosomes.
