Biology 2382B Lecture Notes - Lecture 2: Differential Interference Contrast Microscopy, Confocal Microscopy, Fluorescence Microscope
10 Feb 2018
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Fluorescence microscopy: bright-field microscopy and resolution, phase-contrast microscopy, differential interference contrast microscopy, gfp and fluorescent fusion proteins, confocal microscopy, deconvolution, electron microscopy. Anton van leeuwenhoek (1632-1723): built many simple, single lens microscopes, focused on the composition of a single drop of some sort of fluid, ex. Resolution of microscopes: resolution: the ability to distinguish between two very closely positioned objects as separate entities. Important when seeing detail: a conventional light microscope usually cannot resolve objects/cellular features that are less than ~0. 2 mm or 200 nm apart, need higher power microscopes (electron microscope) Resolution = d: distance resolved between 2 points. Oil: a: 1/2 angle of light entering objective, the limit of resolution is 0. 2 mm=200 nm. Nsina: use a small wavelength of light (small on top, use a substance with a higher refractory index (big on bottom, use a lens that increases the refractory index (big on bottom)
