Biochemistry 2280A Lecture Notes - Lecture 12: Rna Splicing, Polyadenylation, Exonuclease
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QUESTION 1
In the bacterial lac repressor, which of these occurs: (select all that apply)
(a)In the presence of glucose and the absence of lactose, the repressor is actively produced. | ||
(b)The lac operon can be hyper-induced in the presence of low glucose by the 'cAMP binding protein' (CAMP). | ||
(c)In the presence of lactose and the absence of glucose the lac operon is repressed. | ||
(d)Allolactose is a byproduct of β-galactosidase, indicating high lactose when it builds up. |
1.5 points
QUESTION 2
In eukaryotes the enhancer elements can be quite a distance upstream or downstream from the promoter, and transcriptional regulation is helped by protein:protein interactions.
True
False
1.5 points
QUESTION 3
The prokaryotic promoter contains a TATA box that is at -10bp upstream from the transcriptional start site.
True
False
1.5 points
QUESTION 4
During the addition of the poly A tail which of these occurs:(select all that apply)
(a)The transcript is synthesized beyond the poly A site (AAUAAA). | ||
(b)The mRNA contains a 5â cap. | ||
(c)The transcript is cleaved 35nt downstream from the poly A site. | ||
(d)An enzyme complex containing an exonuclease and polyadenylate polymerase cleaves nearby the AAUAA site. | ||
(e)100-300 Aâs are added to the 5â end. |
If one is testing a goodness of fit to a 9:3:3:1 ratio, how many degrees of freedom would be associated with the Chi-square?
1
2
3
4
not enough information to determine
Which of the following choices does not describe a type of posttranslational modification of proteins?
The amino terminal methionine may be removed. |
Glycoproteins are produced by the attachment of phosphates to newly synthesized proteins. |
Precursor proteins are cleaved and trimmed by protease enzymes to produce a functional protein. |
Molecular chaperones help proteins fold correctly. |
Amino acids in the amino terminal of the protein can be acetylated. |
A eukaryotic DNA sequence that affects transcription at distant promoters is called a(n)
long distance promotor |
regulator |
operon |
enhancer |
DNA methylation may be a significant mode of genetic regulation in eukaryotes. Meythylation refers to
altering RNA polymerase activity by adding methyl-groups |
changes in DNA-DNA hydrogen bonding |
altering translation activity via methylated tRNAs |
adding methyl groups to certain cytosines in DNA |
If a nucleotide lacks a 3â-OH group, what will happen?
itâs transformed into RNA |
another nucleotide can bond at the 3â site during replication |
replication will halt |
no effect |
Which of the following describe posttranscriptional modifications commonly seen in mRNA of eukaryotes?
5â capping, 3â-poly-A tail addition, splicing |
3â capping, 5â poly-A tail addition, splicing |
heteroduplex formation |
5â capping, 3âpolyA tail addition, removal of exons |
In birds, females are the heterogametic sex. This means that
females have chromosomes in pairs. |
emales produce eggs. |
females determine the gender of their offspring. |
males have two identical sex chromosomes (ZZ) An anticodon on a tRNA has the sequence 5'âGCAâ3'. What would be the effect on translation if the G in the anticodon were mutated to a U?
|