BIOL 3581 Lecture 4: Biotech Lecture 4

• School: University of Windsor
• Department: Biological Sciences
• Course: BIOL 3581
• Professor: Vacratis

Document Summary

Use purified protein for various applications: enzyme assays, therapeutic assays, used to make antibodies against protein of interest, structure/function studies. Do it under conditions that allow for native purification. When you isolate a protein under native conditions or non-denaturing conditions, the goal of the scenario is to isolate the protein so it has full activity: full quaternary state. If it is a tetramer, you want to isolate the tetramer. Chromatography step will maintain the protein in its fully active state unless it has some specific issues. For pten, it was important for enzymatic activity, structural determination, and ultimately contributing to providing functional clues on the architecture of the protein and the structural mechanism of the enzyme. In terms of historical contribution of this technique: routine, advent of this technology spear-headed a burst of knowledge in structural/functional area of research. Take liver form cows and focus their attention on these enzymes.