PHC320H1 Lecture 7: Lecture 7 Genetics and Technology Impact
Document Summary
Chemical biology: uses tools of chemistry to better understand biological processes, overcome biological problems. Small molecule/gene mutation inhibits protein that is responsible for biological. Small molecule inhibitors provide: control over the level of protein activity, control over timing of protein, transferable reagent, potential drug lead. Classical genetics: forward: random mutagenesis select mutation with phenotype of interest , reverse: mutate gene of interest generate cells with mutation look for identify mutated gene phenotype change. Solutions: hydrophoicity: hydrophobic molecules into membranes (passive diffusion, active transport: protein carriers, but important as drug resistance mechanisms, passive transport: between cells: gap junctions, tight junctions. Yko (yeast knockout) collection: specific for model organism system, mutagenize organism, grow organism on drug, screen for mutation that don"t exhibit drug induced phenotype (suppressors, clone genes for candidate interactors. 2nd test to prove suppressor is target (in vitro assay: elegans diploid transparent survives freezing small hermaphroditic. 3 day life cycle high biological conservation to higher animals.