CSB353H1 Lecture Notes - Lecture 11: Agrobacterium, Chitinase, Wild Type
28 Apr 2016
School
Department
Course
Professor
Document Summary
May introduce non-desirable genes that are tightly linked to target gene. Construct binary plasmid containing t-dna with multiple r genes. Multiple resistance in a single transgenic line cross with susceptible cultivar. Examples of genes that can be introduced to plants for pathogen resistance. Co-expression of glucanase (pr-2) and chitinase (pr-3) genes in tobacco plants. Inducible promoters introduce avr gene hooked with pathogen inducible promoter (inducible by broad spectrum of pathogens) in plant cell, promoter is activated and avr transgene is produced broad spectrum resistance can be conferred. R gene will be activated and induce eti. Pathogen-inducible pr2 (bg2) promoter was introduced in wt cngc gene; conferred resistance. A panel of monoclonal antibodies was raised against virus a specific antibody was found gene that encodes this antibody was cloned from hybridoma cells cloned gene was transferred into plants. Transgenic plant has strong reduction in inoculated and systemic leaf.
