BCH311H1 Lecture : Lecture notes
Document Summary
Rna take information out of dna into more functional form. Rna turned into proteins that trigger cell functions atoms interacting. Miescher - phosphorus-rich material came out of nucleus thus named nuclein. 1 to 1 to 1 to 1 ratio of a, g, t, c. Pauling s idea of triple helix, despite terrible electrostatics. Important placement b/c need right hydrogen bond: differ in placement of hydrogen atom, acceptors and donors. Cells duplicate by generating two dna molecules from one. Three hydrogen bonds - stronger affinity ergo gc-rich dna stronger structure. Melting analysis - heat two strands of dna - strands come apart, at specific temperature (melting temperature) If melting temperature goes down, dna has been destabilized. If melting temperature increases, dna has been stabilized. 260 absorbance increases if heat dsdna b/c when bases stacked up, electrons interact with one another; this interaction causes absorbance at 260 to decrease; when strands apart, more flexibility, this interaction goes away, abosrbance increases.
