BCH311H1 Lecture 1: Nucleic Acid Structure

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Bch311h1s lec01-nucleic acid structure: tetranucleotide hypothesis: levene/tipson, single-stranded dna structure: astbury/bell, discovery of h-bonds (gullard) Base tautomers: enol form is a h-bond donor. Triple-helix structure with phosphate backbone inside (completely wrong: chargaff. A:t = 1:1: dna double helix (watson, crick, wilkin, franklin) Bch311h1s lec01-nucleic acid structure: forces that stabilize dna structure, hydrogen bonding. Canonical watson-crick base pairs: sugars are far apart. Hoogsten base pair: sugars are closer to each other, guanine h bonding in telomere, guanines h-bonding with themselves forming a g-quadruplex. Wobble base pairs: u and g don"t usually base pair, but if they come into the right orientation, they will base pair. Melting temperature (tm) reflect dna stability: tm = half of the sample is dsdna and half is ss dna. Dipole interaction between stacked bases stabilized dna structure. Also affect tm less dipole effect less energy needs to separate strands, tm decreases.

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