BCH210H1 Lecture Notes - Lecture 6: Beta Barrel, Beta Sheet, Solvent

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BCH210H1 Full Course Notes

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Detergents can be used to extract proteins. Salts can be used to disrupt peripheral proteins: change charge on outside on peripheral proteins, peripheral proteins interacting electrostatic binding to membrane, changing charge, be able to remove them using salts to compete and ph to change pka. Organic solvents can be used to solubilize lipids: different compositions of lipids, using organic solvent denature proteins. Integral (intrinsic) cross the membrane (red: single spanning one transmembrane helix, type i & ii, cross membrane once, n-term on outside (type i, n-term on inside (type 2, multispanning 2-24 transmembrane helices. Looping back and forth across membrane: beta barrels multiple beta sheets come together. Lipid anchored fatty acid chains or gpi anchors are covalently attached to protein (blue: to remove: use detergents or cleave off lipid tail to release protein. Sugars are covalently linked to a loop of the protein on the external face of the protein: always found outside of protein.

Related Questions

Hybridomas are the useful result of a fusion between _______________ and _________________ to obtain hybrid cells that can proliferate and produce _________________ against specific ________________.

	myeloma cells; B lymphocytes; monoclonal antibodies; epitopes
	myeloma cells; T lymphocytes; polyclonal antibodies; antigen
	T lymphocytes; B lymphocytes; monoclonal antibodies; antigen
	T lymphocytes; B lymphocytes; polyclonal antibodies; epitopes

With respect to mammalian cell culture, which of the following statements is true?

	Media used for cell growth contain proteases and a divalent cation chelator to help cells adhere to the surface.
	During the establishment of a cell line, senescence is the last stage of the cell's life.
	Rodent cells cultures undergo spontaneous transformation with similar frequency to human cell cultures.
	Embryonic cell lines do not undergo senescence nor do they have a finite life span.

Epithelial cells have distinct surfaces. Which of these surfaces tends to be involved in secreting proteins out of the cell and into the bloodstream?

	apical
	basal
	All of the answers are correct.
	lateral

Which of the following is NOT true of phospholipid bilayers?

		Phospholipid bilayers separate the cell from the exterior environment as well as from compartments within the cell.
		The interior of phospholipid bilayers is a highly hydrophobic environment.
		Membrane budding requires transient breakages in the bilayer membrane.
		The chemical properties of phospholipids lead to the spontaneous formation of bilayers.

Which of the following is true regarding membrane proteins?

		All peripheral membrane proteins contact the lipid bilayer by covalent attachment to the hydrophilic head groups on membrane lipids.
		Beta strands can assemble into a membrane-spanning barrel structure.
		Lipid-anchored membrane proteins always face the cell exterior.
		A protein with six hydrophobic helices is most likely a lipid-anchored membrane protein.

Which of the following statements is NOT true?

		The three types of lipid anchors responsible for tethering proteins to the bilayer are acylation, prenylation, and ubiquitinylation.
		Movement of lipids from the cytoplasmic to the exoplasmic leaflet of the membrane bilayer requires specialized transport proteins called flippases.
		Glycoproteins are the basis for blood type compatibility between donor and receiver.
		Glycoproteins are always oriented so that the carbohydrate moieties are exoplasmic.

BCH210H1 Lecture Notes - Lecture 6: Beta Barrel, Beta Sheet, Solvent

BCH210H1 Full Course Notes

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