BCH210H1 Lecture Notes - Lecture 15: Peripheral Membrane Protein, Integral Membrane Protein, Intracellular Ph

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Sian Patterson

Document Summary

Types of membrane protein classes: unique properties of integral proteins. Extracellular, intracellular, transmem: predicting transmembrane sequences. Types of membrane protein classes: a and b = integral (intrinsic, transmembrane, c and d = peripheral (extrinsic) Peripheral membrane proteins: adhere to membranes or integral membrane proteins through non-cov interactions, operational def"n: removal with mild disruption such as with high salt or small change in ph. Assoc with the membrane --> either sit in, on, or attach to membrane. For this course, this is how to identify these proteins: note the types below, removal of these proteins are done by: High salt --> disrupts ionic interactions and h bonds by shielding charges. Integral membrane proteins: completely span the bilayer and is thus anchored, require harsh conditions to remove the membrane --> detergents / organic solvents, there are four different types of this. Iron channels, receptors, gpcrs, 70% of targets in the pharma industry. Need to destroy the membrane in order to remove.