BCH210H1 Lecture Notes - Lecture 7: Covalent Bond, Protein Kinase, Aspirin
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BCH210H1 Full Course Notes
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We need to have the substrate present or accessible for the reaction to occur. Phospholipase a2 cleaves the c off of the backbone. It"s a (cid:448)er(cid:455) i(cid:373)porta(cid:374)t fatt(cid:455) a(cid:272)id (cid:271)e(cid:272)ause it"s the o(cid:374)l(cid:455) substrate for cyclooxygenase. Addding a phosphate induces a change in the protein because its negatively charged, and normally theres (cid:373)ore tha(cid:374) o(cid:374)e phosphate that"s added, a(cid:374)d the(cid:455) are (cid:272)o(cid:373)(cid:373)o(cid:374)l(cid:455) added to seri(cid:374)e a(cid:374)d threo(cid:374)i(cid:374)e and tyrosine. They are added by protein kinase, and they are removed by a phosphatase. Rate of es dissociation = (k-1 + k2)[es]. The rate of es formation = rate of es diccosiation. Question: its not product concentration, its substrate concentration. To get very accurate values for vmax and km, we use the lineweaver burk plot, which is the reciprocal of the graph. If the enzyme is saturated, its vmax = kcat[et]. Rate oof product production, when all enzymes are occupied by sunstrate, kcat = vmax/et.