NROB60H3 Lecture Notes - Lecture 1: Sheep, Lactate Dehydrogenase, Buteo
Document Summary
Final exam review questions: describe three differences between dna sequencing performed using classic sanger sequencing and next generation pyrosequencing. Sotring and growing enormous numbers of bacteria. Dna purification for every chunk of genomic dna. Must have 1 pcr tube for each reaction. Must have 4 lanes for every sequence reations. Simultaneously sequence millions of bp at a time. Use the photon emitted by different nucelotides and a camera to pick up the energy to sequence the dna- after placed in well and after being amplified on beads. Shot gun sequencing by pgm/454: add adaptors of known sequence to genomic gragment- addapotors= little pieces of. Over all- sanger method= selective incorporation of chain terminating dideoxynucleotide by. Dna polymerase during in vitro dna replication- in test tubes using bacteria- pcr takes place in test tube and no beads need bacteria to grow dna: reaction mixture- primer/ dna template. ddntps with flourochromes.
