BIOB11H3 Lecture Notes - Lecture 8: Methionine, Consensus Sequence, Alpha And Beta Carbon
Document Summary
Have sharp edges on them (sickle cells) Ingram purified hemoglobin from normal vs. sickle cells and looked at their sequences = single amino acid change in a protein caused this disease. Figure 11-41: non-synonymous mutation = different aa produced, frameshift mutations occur due to indels. Multiples of 3 mutations results in an insertion and the rest of the following chain still encodes the same aas restores the reading frame. Help to elucidate the code = new tool, brute force, brilliance (3 categories: (1) new tool in vitro = in a test tube. Harvest cells, lyse cells, release rna, atp + other components needed for translation. Radioactive aas added = radioactive protein is produced. Graph = nothing happens, add aa*, add rna that will associate with ribosomes = gives you more radioactive proteins (assuming ribosomes aren"t already saturated) sudden increase: (2) organic chemist. Bacterial extract, added poly u/a/c which yielded different aas.
