BIOB11H3 Lecture Notes - Lecture 4: Tata-Binding Protein, Transcription Factor Ii D, Polyadenylation
Document Summary
Mrna synthesis: hnrna is the precursor to the mrna, synthesized in nucleus, heterogenous nuclear rna (hnrna) is processed into smaller rna molecules and exported to cytoplasm, mrna and hnrna precursor rapidly degraded half life in minutes overall conclusion hnrna is precursor to mrna, rna polymerase ii synthesizes all eukaryotic mrna precursors. Several different subunits: general transcription factors (gtfs) (cid:224) general because their binding is required for transcription to initiate at many different promoters, pol ii recognizes promoter regions located towards the 5" side of the transcription unit, tata box. Tata binding protein (tbp: recognizes the tata box, required for rna pol ii to initiate transcription at the correct site, tbp binds in minor grove of dna and alters its conformation at site of dna protein interaction, part of large protein complex , tfiid ( transcription factor for polymerase ii, fraction d) consists of 38 kda tbp and.
