CHM362H5 Lecture Notes - Lecture 22: Glycogen, Polysaccharide, Insulin

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2 Jan 2016
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Glycogen metabolism: chm361 review, glycogen storage polysaccharide made up entirely of glucose molecules, glucose molecules connected by (1,4) links with (1,6) branches, (1,6) branches every 8-12 residues. Digestion of amylopectin and glycogen: dietary amylopectin and glycogen digested by amylase enzymes, -amylase is an endoglycosidase that is found in saliva and pancreatic juice, -amylase cleaves amylopectin and glycogen randomly at (1 4) linkages. Glycogen synthesis: requires activated glucose in form of sugar nucleotide udp-glucose, udp-glucose pyrophosphorylase catalyzes a phosphoanhydride exchange, driven by pyrophosphate hydrolysis. Glucose-1-p + utp + h2o udp-glucose + 2 pi. Glycogen synthesis, cont"d: the very large glycogen particle is built around a glycogenin core, the first glucose is linked to a tyrosine -oh on glycogenin, sugar units are then added by the action of glycogen synthase. Transfer of glucose from udp-glucose to c-4 oh at non-reducing end of glycogen. Regulation of glycogen metabolism: glycogen synthase should be active when glycogen phosphorylase is inactive.

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