BPS 3101 Lecture Notes - Lecture 7: Oligomer, Concatemer, Genotyping

• School: University of Ottawa
• Department: Biopharmaceutical sciences
• Course: BPS 3101
• Professor: Linda Bonen

Document Summary

Assaying molecular barcode tags in yeast pools: yeast deletion strains with barcodes up & downstream of inserted gene from vector. So if deletion of gene x is lethal under certain growth conditions no pcr product. Functional profiling of saccharomyces cerevisiae (yeast ~6000 genes) genome: 5916 deletion mutant most show no major phenotypic effect. Need to look at more conditions (changes in morphology, maybe another gene can compensate for knock-out of gene x) Function redundancy: used to describe situations where a given biochemical function is redundantly encoded by two or more genes. In these cases, mutations (or defects) in one of these genes will have a smaller effect on the fitness of the organism than expected from the genes" function. E. g if gene x is a member of a multi-gene family: insertional mutagenesis: Transposon tagging: if transposon inserts into gene (or into regulatory seq)