BCH 4101 Lecture Notes - Lecture 12: Crispr, Cas9, Ctcf
12 Dec 2019
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Still, have loops that form even without the dot, it just means that it"s not ctcf mediated. Be able to recognize all the points and elements of hi-c for final. Trans - interactions between the chromosomes are rare. Chromosomes will preferentially interact with sequences within themselves rather than other chromosomes. This phenomena + other fish studies prove that chromosomes occupy their own area. Individual chromosomes occupy distinct territories within the nucleus. Modify crispr to create something that can change the folding or spatial organization of the genome. What happens to gene expression when we move something that"s at the center of the nucleus towards the periphery. Will it become silenced or stay active. Coupling of crispr-dcas9 system with nuclear-compartment-specific proteins. Coupled the dead cas9 with half of a heterodimer. Abi + pyl1 (2 halves of a dimer) In the presence of the ligand, those two proteins will dimerize. Take 1 half of the heterodimer and fuse it to cas9 (abi)
