BCH 4101 Lecture Notes - Lecture 11: Fibrillarin, Fluorophore, Nucleolus
12 Dec 2019
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Document Summary
Dna sequences the variations and the impact of this. How these nucleotides are organized into gene units. What controls the expression of the gene. How the dna is organized into the chromosome and how they fit in a nucleus. In the beginning, we didn"t know what was inside the nucleus - used electron microscopy. Need tools to look inside the cell. Know there"s a dark stained spot in the middle. Now we know is the nucleolus: site of ribosomal. Ordered internal st4ructur related to the variety of biochemical activities. Very structured - and related to the activities going on inside the cell. 2nd stage was the development of different fluorophores. And tag different components inside the cell and see where they localize. Better understanding with the advent of fluorescent markers. If we use blue fluorophor and use it to tag fibrillarin. We see that the blue fluorescents clusters in two distinct areas inside the nucleus.
