CAPS 390 Lecture Notes - Lecture 3: Vesicle Fusion, Clathrin, Lipid Bilayer Fusion
23 Nov 2015
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Membrane stack is stationary and cargo moves between cisternae in vesicles that bud from one and fuse to the next. Cisternae move with cargo and mature through stack and are consumed by packaging in tgn. Golgi enzymes are recycled back to appropriate compartments in vesicles. Problems: golgi enzymes not seen in golgi vesicles; cargo moves through stack at different rates. Receptor-mediated endocytosis: lysosomes, non-vesicular lipid traffic. Large protein cages that are spherical in shape. 1) recruit cargo and 2) drive vesicle formation. Snare: alpha helical membrane protein that interacts to drive membrane fusion v-snare: on vesicle membrane t-snare: on target membrane. Vesicle targeting and fusionmembrane fusion - snaressimilar to fig 2-22. Membrane traffic: is coupled process of exocytosis and endocytosis exocytosis results in addition of membrane endocytosis balances membrane addition from exocytosis. Phagocytosis - large particles, e. g. macrophages ingesting bacteria. Pinocytosis - fluid, uses small vesicles (< 150 nm), accounts for most of the endocytosis in most cells.
