CHEM237 Lecture Notes - Lecture 1: Proline, Phenylalanine, Edman Degradation
6 Oct 2017
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6m hcl, ion exchange since it"s 6m (so high) it will protonate it and hydrolyze all the amide bonds and make all the amino acids positively charged. What"s going to happen to glutamine under these strong acid conditions, since. So you will give a tyr, leu, pro, 2 glu, lys, and one molecule of nh3. Fdmd an n terminal reagent so it will react with the n terminal. Fdmd reacts with the amine function of tyr. The nitrogen is not nucleophilic because it is resonance stabilized. Chymotrypsin (a protease) strong specificity for aromatic rings so it cleaves right after the aromatic ring, which in this case is only tyrosine. You would thus get a single tyr, a 5-mer (a pentapeptide). One amino acid and then the pentapeptide; you should know that it could be: tyr _ _ _ _ _ (1 + 5, _ _ _ _ tyr _ (1 + 5)
