BIOL309 Lecture Notes - Open Reading Frame, Dna Supercoil, 16S Ribosomal Rna
16 Mar 2013
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Document Summary
Biol 208 analytical methods of molecular biology. 5" p links one sugar to 3" position of next. Degradation of dna require nucleases that break covalent bonds . Greater susceptibility to degrade by ribonucleases chemical degradation by alkaline conditions. Strands are antiparallel running 5" (phosphate) to 3" (hydroxyl) in either direction. Dna easily denatured h bond breaks without affecting sugar-p. Gc (3 h bonds) melt less easily than at (2 h bonds) Estimate tm -> known sequence and buffer conditions. Dna structure maintained by hydrophobic interactions between bases ssdna/rna unstable in water. Secondary structures of ssdna/rna: hairpins, stem-loop difficult to separate strands plays role in gene expression initiation of dna replication. Negative charge on dna backbone causes electrostatic repulsion. Presence of salt neutralizes (-) charge on p groups. Reduction of [salt] weak interactions between strands. Nucleotides always added to growing end (3" oh) Substrate is dntp in dna and ntp in rna. Types of circular forms (a) closed circular form.
