BIOL240 Lecture Notes - Lecture 11: Transposable Element, Dna Sequencing, Transposase
1 Feb 2018
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Uses of conjugation: triparental conjugation, conjugation can still happen -> recombinant plasmid -> lacking tra gene and helper plasmid -> with tra gene = more room in recombinant, think of two plasmids fusing together. Transposition: movement of dna -> mobile genetic elements, transposons, genes needed for transposition. Transduction: foreign dna is introduced into host via virus/vector, virus -> accidentally packages host genome -> delivers host genome -> ca(cid:374)"t replicate -> homologous recombination -> exchange of dna strands -> (cid:862)switchi(cid:374)g it up(cid:863) Topic 10 genomics: genome: all genetic information, genes, regulatory sequences, non-coding sequences, genomics: discipline involving working with genomes, sequencing, mapping, analyzing, (cid:862) tud(cid:455) of ge(cid:374)o(cid:373)es(cid:863, functional genomics: assigning function to unknown genes. Pyrosequencing: detects addition of nucleotide to end of strand with light, luciferase -> light, faster, cheaper than sanger, do(cid:374)"t (cid:374)eed to be cloned -> only requires fragmentation.
