BIOL 2520 Lecture Notes - Lecture 5: Nucleoplasm, Karyopherin, Fluorescent Tag
1 Dec 2016
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Document Summary
Proteins enter the nucleus through nuclear pores 495-497. Nucleus: storage and decoding of genetic information. Contains dna: condensed and organized with proteins as chromatin. Nuclear matrix: protein containing fibrillar network. Nucleolus (pl. nucleoli) rrna synthesis, ribosome assembly surrounded by nuclear envelope (all above is) Chromatin: active chromatin (euchromatin) appear lighter. Euchromatin is an active chromatin where all genes are transcribed. Inactive chromatin (heterochromatin) is darker and located near periphery of nucleus. Heterochromatin are tightly packed not allowing for transcription: fig to the right shows interphase nucleus. Nucleolus: a granular region, site of ribosome assembly. Two lipid bilayer, membranes 10-50 nm apart: the outer membrane is continuous with endoplasmic reticulum, dense layer of intermediate filaments, the nuclear lamina, is within the inner membrane. Provides structure to nucleus, heterochromatin binding site. Defect in lamin a gene results in progeria- premature aging. This is the lamin a in progeria nucleus (causes deformation) These nuclear import receptors can then bring in the protein.
