MCB 2050 Lecture Notes - Lecture 1: Schizosaccharomyces Pombe, Glycoprotein, Microbiological Culture
8 Mar 2021
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Lab 1 notes: cerevisiae: asexual reproduction by multilateral budding & non-motile, pombe: asexual reproduction by fission & non motile. Cell morphology: large rod and single arrangement purple: gram-positive reaction purple bc they retain crystal violet-iodine precipitate in their thick peptidoglycan mesh (pm) and resists de-staining by alcohol. Cell morphology: short rod and single arrangement pink/red: gram-negative reaction pink bc alcoholc de-stains crystal-violet precipitate from the thin peptidoglycan mesh. Safranin dye (pink) is added to counter stain the colourless cells in last step of gram protocol outer cell membrane and thin peptidoglycan mesh of gram-negative bacteria are unable to resist de-staining by alcohol. Cell morphology: rods, arrangement as single to short filaments, somewhat pleomorphic in cell shape pink: gram-negative reaction. Archaea cell walls have diverse chemical and structures; they don t contain peptidoglycan in their cell wall but some have pseudomurein (which is a type of peptidoglycan in their cell wall & retain crystal violet-iodine precipitate thus giving a.
