MCB 2050 Lecture Notes - Lecture 5: Western Blot, Surface 3, Two-Dimensional Gel Electrophoresis
20 Oct 2016
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Document Summary
Comparative genomics: the study of genome evolution and comparison of genomes of various organisms. Uses bioinformatics techniques to: deduce structure/function of gene products, create phylogenetic trees based upon dna sequence comparisons to study chromosomal genome evolution. Bioinformatics: the study of genetic and other biological information using computer and statistical techniques. Given a nucleotide sequence, you can: find open reading frames, deduce amino acid sequences, find homology to other proteins, find common domains. Synteny: conservation f relative gene/marker chromosome and order. Tanscriptome: entire set of mrna transcripts produced by a given organism. Changes in transcriptome with development, time, tissue, cellular signals, environmental insults, virus infection. Proteome: the entire array of proteins encoded in an organism. Used to measure gene expression on a large scale. Knowing the sequence of all human genes makes it possible to study the expression of large subsets of genes in a singe organism. Spotted microarrays cdna fragments or synthetic oligonucleotides are spotted or (cid:498)printed(cid:499) onto a.
