CHEM 1050 Lecture Notes - Lecture 37: Molecular Mass, Barium, Bismuth
24 Dec 2016
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Ab+ and a+ are the fragments these are useful for sequence determination sequence determination i. e. primary sequence of a peptide. Mass spectrometry is useful to define the molecular mass and sequence of a biomolecule. **exam question: ionization sources in ms (mass spectronomy): 1. electron impact (like in g. c. -ms) fire electrons at your sample, so your sample molecules break apart, then a mass spectrometer analyzes the mass to charge ratio and identifies what"s in there. Usually for liquids spray the liquid out a nozzle in a really fine spray - the nozzle is charged, so the particles are ionized, and a mass spectrometer analyzes it. Protein sample embedded in a resin, then it"s hit with a laser - the proteins turn into their gas phase, and the gasses are then sent through the mass spectrometer. Nuclear-magnetic-resonance (nmr) some nuclei you can detect by nmr. **exam question: the 4 below are the nuclei you can detect.
