BIOC 4580 Lecture Notes - Lecture 28: Chondroitin Sulfate, Mns Antigen System, Heparan Sulfate
23 Mar 2017
School
Department
Course
Professor
Document Summary
We have already discussed carbohydrate attachment to membrane proteins & how this moiety ends up extracellularly indeed, it forms a glycocalyx coat. The erythrocyte glycocalyx is due to large carbohydrate chains on glycophorin (determining blood group types) and small ones on band 3. Erythrocyte glycosphingolipids also contribute to blood group types. Most other cell types have an extracellular matrix derived from glycosaminoglycans, proteoglycans, fibronectins, & integrins. Lectins (carbohydrate-binding proteins, with antibidy-like specificities) & glycosidases (digestive enzymes) are useful tools in glycomics, followed by mass spectrometry and/or nmr spectroscopy. Carbohydrates (oligosaccharide chains) are asymmetrically located on outer surface of plasma membrane, lumenal surface of er, golgi, etc. , in form of hybrid glyconjugates: Neutral glycolipids (ceramides cerebrosides & globosides) gangliosides. Macromolecules of cell surface or extracellular matrix (ecm) with protein core and sulphated glycosaminoglycan chains. Eukaryotic cells have a fuzzy glycocalyx ( sugar coat ) Have talked about the membrane being an impermeable barrier and a capacitator holding a charge across it.
