BIOC 4580 Lecture 8: Membrane Protein Topology - Bioinformatics & Molecular Biology
3 Feb 2017
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Membrane protein topology bioinformatics & molecular biology. Since electron and x-ray crystallography are difficult and require dedicated resources, they cannot be performed by just any laboratory. Not only that, crystals might prove elusive. Thus, bioinformatics approaches are a first tool to help understand a membrane protein"s topology. Such predications can be used to guide molecular and cell biological experiments to probe portions of the protein that are within the membrane, or exposed on the cytoplasmic or ec surfaces. These experiments involve genetic manipulation and fluorescence microscopy of transfected cells. Biophysical approaches for the determination of tmp structure. Can be used to predict segments that are transmembrane along with a host of other data. Where are the termini, tm segments, b-turns & loops. Every aa has a certain hydrophobicity quantity with it, based on: (i) (ii) (iii) Experimental data relative solubility/partitioning of aa"s in an organic solvent vs. aqueous sol"n.
