BIOC 2580 Lecture Notes - Lecture 11: Competitive Inhibition, Covalent Bond, Cytochrome C Oxidase Subunit Ii
10 Feb 2018
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Lecture 11 linear plots and enzyme inhibition. 11. 1: the michaelis-menten equation is a hyperbolic curve when vo is plotted vs [s], and approaches. Vmax gradually: real experiemental data often showers scatter due to measurement errors, lineweaver-burk method, linear transformations convert michaelis- Menten equation into a straight-line form: take reciprocals of both sides of. Michaelis-menten equation: use 1/vo for y and 1/[s] for x, slope = km/vmax, y-intercept = 1/vmax, x-intercept = -1/km. Inactivation results from covalent chemical reaction between inactivator and enzyme: often irreversible reaction destroys catalytic activity and uses up enzyme, simple stoichiometric relationship between inactivator and enzyme, 2 micromol inactivator + 3 micromol enzyme. = 1 micromol active enzyme left: many inactivators are highly toxic nerve gases inactivate the enzyme acetylcholinesterase, interfering with nervous impulses. Inhibitors can regulate enzyme activity in the cell; more economical for cell to make and destroy a small inhibitor than a large enzyme: many drugs are enzyme inhibitors.
