BIOC 2580 Lecture Notes - Lecture 17: Equilibrium Constant, Hmg-Coa Reductase, Non-Competitive Inhibition
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More economical for cell to make and destroy a small inhibitor than. E formation of ei complex means less e available to bind substrate inhibitor and substrate compete for available enzyme - high [s] can overcome competitive inhibitor. K i is a characteristic constant for each inhibitor (like k m ) K m " increases as [i] increases so x intercept gets smaller slope increases as [i] increases; y-int = 1/v max x-int = -1/k m ". V" max = v max / (1 + [i] / k i ) K m is unchanged (mixed inhibition may show a small effect) if [i] is set to equal k i , inhibition factor = (1 + 1) = 2. V" max decreases as [i] increases, so the y-intercept gets bigger slope increases as [i] increases x-int = 1/k m y-int = 1/v" max.