BIOC 2580 Lecture Notes - Lecture 18: Linus Pauling, Beta Sheet, Bond Order

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: Enoka Wijekoon

Document Summary

First section of the machine (called "ms-1") separates peptides by mass. Move into collision cell where each fragment will be broken into 2 fragments (usually at a peptide bond) randomly (any peptide bond along the chain can be broken) *each fragment going into the mass spectrometer must have a charge for the mass spectrometer to recognize it. Generated with trypsin at a low ph, so the peptide has an arg which is. Once the fragment has been split in 2 parts, ms-2 measures the mass of the charged fragment. Between at all the copies, each different peptide bond along the chain will be broken, thus by finding the differences in masses you can calculate the mass of each amino acid. <156 = mass of arg weighs sequence starting at the c-terminal end (starting with arg) since this was the charged end, but make sure you write the sequence starting at the n-terminal end.